Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig
Identifieur interne : 002909 ( Main/Exploration ); précédent : 002908; suivant : 002910Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig
Auteurs : Na Young Kim [Corée du Sud] ; Donghee Lee [Corée du Sud] ; Jungeun Lee [Corée du Sud] ; Eung Woo Park [Corée du Sud] ; Woon-Won Jung [Corée du Sud] ; Jai Myung Yang [Corée du Sud] ; Young Bong Kim [Corée du Sud]Source :
- Virus Genes [ 0920-8569 ] ; 2009-10-01.
Descripteurs français
- Wicri :
- topic : Zoonose.
English descriptors
Abstract
Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.
Url:
DOI: 10.1007/s11262-009-0377-7
Affiliations:
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xml:lang="fr"><term>Zoonose</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Xenotransplantation from pigs offers an opportunity to resolve the shortage of human organs. The porcine endogenous retrovirus (PERV) cannot be eliminated because of its presence in the germline DNA. Three subgroups of the replication-competent PERV (PERV-A, PERV-B, and PERV-C) have been identified in pigs. We constructed a molecular clone of PERV-B from a Korean domestic pig BAC clone containing PERV genomes, and its replication competency was characterized in human cells. The pol region of PERV-B was detected in the genomic DNA of 293T cells transfected with PERV-B (465D1) and in the genomic DNA of 293T cells infected with PERV-B (465D1) viruses. The 293T cells transfected with PERV-B (465D1) were maintained for 140 days. PERV-B (465D1) showed the stationary replication competence with no toxicity in the cell growth.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Kim, Na Young" sort="Kim, Na Young" uniqKey="Kim N" first="Na Young" last="Kim">Na Young Kim</name></region><name sortKey="Jung, Woon Won" sort="Jung, Woon Won" uniqKey="Jung W" first="Woon-Won" last="Jung">Woon-Won Jung</name><name sortKey="Kim, Na Young" sort="Kim, Na Young" uniqKey="Kim N" first="Na Young" last="Kim">Na Young Kim</name><name sortKey="Kim, Young Bong" sort="Kim, Young Bong" uniqKey="Kim Y" first="Young Bong" last="Kim">Young Bong Kim</name><name sortKey="Kim, Young Bong" sort="Kim, Young Bong" uniqKey="Kim Y" first="Young Bong" last="Kim">Young Bong Kim</name><name sortKey="Lee, Donghee" sort="Lee, Donghee" uniqKey="Lee D" first="Donghee" last="Lee">Donghee Lee</name><name sortKey="Lee, Jungeun" sort="Lee, Jungeun" uniqKey="Lee J" first="Jungeun" last="Lee">Jungeun Lee</name><name sortKey="Park, Eung Woo" sort="Park, Eung Woo" uniqKey="Park E" first="Eung Woo" last="Park">Eung Woo Park</name><name sortKey="Yang, Jai Myung" sort="Yang, Jai Myung" uniqKey="Yang J" first="Jai Myung" last="Yang">Jai Myung Yang</name><name sortKey="Yang, Jai Myung" sort="Yang, Jai Myung" uniqKey="Yang J" first="Jai Myung" last="Yang">Jai Myung Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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